Neural-Ozone Therapy (NOT) Therapy
Neural-Ozone Therapy (NOT) Subcutaneous Ozone Injections
What is Neural Ozone Therapy?
Subcutaneous ozone therapy – referred to as NOT (Neural Ozone Treatment) at Carolina Integrative Wellness- is an emerging regenerative approach that uses small amounts of medical-grade oxygen-ozone gas injected just beneath the skin around painful and inflamed areas.
This minimally invasive therapy is designed to support local circulation, tissue oxygenation, and cellular signaling in targeted areas. At our clinic, we follow strict sterile protocols, individualized dosing, and careful anatomical assessment to ensure safe and precise administration.
Unlike intra-articular injections, NOT is placed just beneath the skin near nerves and soft tissues.
The goal is to support:
- local circulation
- oxygen delivery
- inflammatory signaling balance
- neuromodulation of pain pathways
Why patients consider it: Some patients seek NOT for localized pain, inflammation, or tissue support when looking for minimally invasive options beyond oral medications or systemic treatments.
Scientific Background
Ozone therapy has been studied for several biological effects relevant to musculoskeletal conditions.
Research suggests ozone may:
- modulate oxidative stress
- influence inflammatory mediators
- improve tissue oxygen utilization
Clinical studies have shown ozone injections improved pain and functional outcomes in osteoarthritis patients. (PLOS)
Meta-analysis data also indicate ozone therapy may reduce pain intensity in chronic joint conditions. (ScienceDirect)
Research in Regenerative Biomaterials (2019) found increased fibroblast activity, improved collagen organization, and higher FGF-2 expression after ozone treatment.
Another study in Journal of Burn Care & Research (2021) demonstrated enhanced healing markers in burn models.
Additional studies in Neuroscience Letters (2009) and Pain Practice (2024) suggest ozone may influence inflammatory mediators and mitochondrial redox balance, supporting potential neuromodulatory effects.
Laboratory and preclinical studies suggest subcutaneous ozone may modulate oxidative stress and inflammatory pathways while supporting tissue repair.
Typical treatment course & what to expect
- Thorough intake and review of imaging (X-ray, MRI) when available.
- Procedure performed in clinic using sterile technique.
- Careful subcutaneous placement of small volumes of ozone gas in targeted areas.
- Short observation period and post-procedure instructions.
Most patients receive a series of injections spaced over several weeks, depending on response.
Safety & who may not be a good candidate
NOT Therapy FAQ
Q. Is ozone safe?
Medical ozone therapy has been used for decades in Europe and other countries. When administered properly it is considered safe.
Q. How quickly does ozone work?
Some patients notice changes within minutes due to neuromodulation effects, while others improve gradually
Q. How many injections are needed?
Frequency is customized depending on response.
Q: Does Neural Ozone Therapy cure pain or inflammation?
A: No therapy guarantees a cure. Studies suggest subcutaneous ozone may support pain reduction, inflammation modulation, and tissue signaling in some patients, but responses vary. (ScienceDirect)
Q: Is the procedure painful?
A: Most patients experience mild local discomfort/burning feeling during injection. These injections are directly under the skin using gentle technique to help minimize any pain.
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